We have developed a portfolio of peptide-ready MHC (prMHC) proteins which are MHC monomers or tetramers stabilized without an antigenic peptide. These MHCs are peptide-ready meaning they can loaded with your custom neoantigen peptide in-house to generate a custom MHC monomer or tetramer.
In neoantigen identification, assessing the immune functionality of the antigenic peptide is critical, which includes evaluating its binding affinity to MHC and its reactivity with TCR. To facilitate this, KACTUS has introduced a range of functional prMHC catalog products. These can serve as a ready-to-use loading system to assist in loading antigen peptides and subsequently form a new, complete MHC peptide complex, thereby significantly aiding your neoantigen research studies by ensuring efficiency and reliability.
Enabling rapid and high-quality creation of custom Class I MHC tetramers, our prMHCs provide a streamlined and user-friendly approach to developing new custom MHC monomers and tetramers in just a few minutes directly in your lab. Generated custom MHC monomers or tetramers can be used to analyze MHC/TCR binding affinity in ELISA, SPR, flow cytometry, etc. This innovative system holds applications across various realms including epitope discovery, neoantigen vaccine research, and verification of T cell staining, among others.
prMHCs can be used for functional screening of peptides for MHC class I binding, a critical component in vaccine design and immune monitoring. They provide the distinct ability to discriminate between MHC binding and non-binding peptides, which is particularly pivotal when screening immunogenic peptides derived from infectious agents or cancer neoantigens. Additionally, the fast and simple peptide-loading protocol makes it faster to create peptide-MHCs when screening large numbers of peptides.